L:19 Observation of starch granules, micro-organism

L:19

Optical microscope (16x10 )cell of carrot 

Optical microscope (16x10) see the potato starch granules stained with IODA

Optical microscopewith oil imersio of  (100x10) see the potato starch granules stained with IODA

Opticaal microscopewith 16x10 cell of carrarot cromoplast

https://youtu.be/CMIXjBLSTFw          

video the organism cells

L:17 Gram staining

L:16

 Introduction:

 In this great experiment we yogurt bacteria stained with dyes to diferend observarlos microscope after.

Objectives:

1- Differentiate yogurt bacterias.
2- Relate the staining procedure with the structure of the cells.

Materials:

- 1 Slide
- 1 Cover slip
- Tongs
- Needle
- Gram stain: Crystal violet,iodine and safranin.
- Decolorize reagents: ethanol 96%
- Microscope
- Yogur

Procedure:

Prokariotic cell observations: Gram Staining

1- Prepare a heat-fixed sample of the bacteria to be stained
2- Cover thesmear with crystal violet for an exposure of 1 min
3- Rinse with distilled water
4- Apply Iodine solution for 1 min.
5- Rinse the sample with distilled water.
6- Decolorize using ethanol.Drop by drop until the purple stop flowing.Wash immediately with distilled water.
7- Cover the sample with the safranin stain for an exposure time of 45seconds.
8- Rinse the sample with distilled water.
9- Gently dry the slide with paper(only the under pard of the slide )



Questions:

Gram Stain:

1.Complete the table thay you have below:

	GRAM+	GRAM-
Crystal violet: Color?	VIOLET	VIOLET
Iodine	Color changes	Color changes
Ethanol Decolorize?	No Dye	Colorfast
Safranin Color?	No Dye	Dyed Pink

Photo the yoghurt bacteria

Microscope optical

http://microbiologiabactesp3.blogspot.com.es/2009_08_01_archive.html

L:16 Epidermis Cells

L:16

Introduccion:

-In this experiment we observed the microscope the epidermis of a plant which was a joint seen the wall and cellular nuclei.

Materials:

- 1 Slide
- 1 Cover Slip
- Distilled water
- Scissors
- Needle
- Join

Procedure:

Plant cells observation

1- Cut the stalk of the leek.
2- In the place of the cut,pullout the trasparent part of the epidermis
3- Using the brush, place the peel onto the slide containg a drop of tap water
4- Take a cover slip and place it gently on the peel with the aid of a needle
5- View it in the microscope.
6- Describe the change in the shape of the cells.
7- Draw a diagram with the parts of a stome: cells,guards,epidermis cells.

Salt Treatement

1- Prepare a 10% of salt solution
2- Put the salt with a dropper on the left pard of the slide .Touch the cover slip.
3- Place a piece of cellulose paper in the opposite part of the cover slip, and let the dissolution to go through your sample.


Resuls and microscope observation.






100x augments
Photo epidermis cells join



Questions 

1- What is the major function of a cell membrane?   Balance of the internal environment of the external environment.

2-What is the major fuction of the cell wall? Performance featuring the contents of the cell and give rigidity, intervenes in all relations with the cell environment.

3-How does salt affect the cells shape?And the stome? Distend plant cell, putting large enough to balance the difference in osmotic pressures that occur solution external and internal pressure. The function of stomata have obrirce and tancarse as the plant that has this great remedy in case closed and tried to regulate the salt environment.